Several azoospermic patients with non-obstructive azoospermia (NOA) undergo for sperm aspiration as an important part of their in vitro fertilization procedure. Sperms may be present in some parts of the testes, however not all the parts, thus, many sampling of the testicular tissue are done for increasing the chance of finding sperm in NOA patients.

With medical advancement there have come several sperm retrieval methods. Sperms can be easily found in more than half men with non-obstructive azoospermia. Micro TESE, microsurgical testicular sperm extraction refers to a procedure that is performed for precise removal of small volume of testicular tissue in the area of active sperm production by using the microscope, thus improving the sperm yield. This particular process has several advantages when compared to other sperm retrieval methods. It maximizes the production of spermatozoa while reducing the amount of testicular tissue needed and vascular injury created by making identification of the blood vessels relatively easier.